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. 2014 Dec;20(12):1963–1976. doi: 10.1261/rna.044354.114

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© 2014 Delvillani et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 7. — Effect of point mutations on PA5194-sfGFP thermoregulation. Cultures of E. coli DH10B carrying plasmids pGM2013 (2013) and its mutated derivatives pGM2013CCC (2013CCC) and pGM2013AAA (2013AAA) were grown at the temperatures indicated below the bars and processed as detailed in Materials and Methods. pGM2016 (2016) was analyzed as negative control and showed modest (less than twofold) thermal induction. Fluorescence emission (F_485/535) was measured in a Packard FluoroCount microplate reader at 485/530 nm. The fluorescence was expressed in arbitrary units (AU) as the ratio F_485/535/OD₆₀₀. The average results of two independent measurements are reported with standard deviations. Relative fluorescence with respect to pGM2013 at 28°C is indicated by the numbers on top of the bars.