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. 2014 Dec;20(12):1987–1999. doi: 10.1261/rna.046532.114

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© 2014 Wroblewski et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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FIGURE 1. — Phenotypes and sRNA profiles in transgenic Arabidopsis ecotype Ws-0 transformed with four constructs configured to produce different ihpRNAs. (A) Predicted ihpRNA structures produced by expression from the integrated T-DNA. (B) Expression of β-glucuronidase in the leaves of transgenic T₁ plants measured after Agrobacterium-mediated transient expression of the uidA (GUS) gene. Each bar shows the average GUS activity per T₁ plant. (C) Percentage of T₂ plants derived from each T₁ plant shown in (B) sorted into four classes depending on anthocyanin production: (red) not silenced, (red-yellow) weakly silenced, (yellow-green) moderately silenced, and (green) strongly silenced. The white rectangle indicates the plants used for construction of sRNA and RNA-Seq libraries. (c) control wild-type plants, Arabidopsis thaliana ecotype Ws-0. Each bar corresponds to 48 or more T₂ individuals derived from each T₁ plant shown in (B). (D) Abundance of transgene-derived sRNA sequences corresponding to the ihpRNA sequences. Roman numerals (purple) indicate peaks of sRNA accumulation compared between libraries. Abundance of sRNA in 1 million reads per library is shown. (*) 20-nt long sRNA accounted for up to 2% of the 20- to 21-nt ihpRNA-derived sRNA shown in the alignment.