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SH intensity versus bulk PnA concentration binding to 5 mol % GM1 doped into a DOPC bilayer recorded at steady-state equilibrium (●), at non-steady-state equilibrium (▲), and to a pure DOPC bilayer (○). Lines represent the fits to the Frumkin binding model (solid) and Langmuir model (dashed). Error bars represent the standard deviation from three independent experiments.
