Figure 1.
Sensitivity of SWI/SNF deletion strains to chemotherapeutic agent doxorubicin.
Notes: (A) The survival of the strains to 20 μmol/L doxorubicin was determined as described in the “Materials and methods” section. Serial dilutions (1:10–1:105) of the treated cultures were spotted onto YPD plates. Growth was scored after 3 days of incubation at 30°C. The serial dilutions of the strains are shown. Positive controls sensitive to doxorubicin are the ssz1 and ydj1 deletion strains. (B) Quantification of the survival of the tested strains. Survival was determined by counting the number of colonies in the respective dilutions and calculated on the basis of the growth of strains not treated with doxorubicin. At least three sets of experiments were used in the statistical analysis. Average survival plus standard deviation is shown. The P-values are indicated for each mutant.
Abbreviations: YPD, yeast extract/peptone/dextrose; WT, wild type; Dil, serial dilutions; Doxo, doxorubicin.