TABLE II. Polymerase chain reaction (PCR) results for hepatitis B virus (HBV)-DNA positivity among the 215 children with clinical hepatitis.
PCR assay | Samples (n) | PCR results | ||||
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Diagnostic-PCR | 215 | 170 -ve | 45 +ve | |||
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Confirmatory OBI-PCR | 45 | 21 -ve (samples) | ||||
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24 +vea | ||||||
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S | C | P | X | |||
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21 | 10 | 5 | 6 | |||
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H-specific-PCR | 24 | H | Non-H | Hb | ||
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17 | 3 | 4 |
a: three samples were hepatitis B surface antigen (HBsAg) positive/HBV-DNA +ve; b: there was not sufficient volume of serum to perform DNA extraction once more to confirm genotype H; OBI: occult hepatitis B infection; -ve: negative samples; +ve: positive samples.