Figure 2.

Reaction scheme showing the proposed inhibitory mechanism of pyruvate carboxylating activity by l-aspartate in the presence of acetyl-CoA, where k₀ and k_cat are catalytic rate constants for the acetyl-CoA-independent and -dependent reactions catalyzed by the enzyme (E) and enzyme·acetyl-CoA complex (EA_h1), respectively. The catalytic rate constant for the reaction catalyzed by the enzyme·l-aspartate complex (EAsp_h2) is k_asp. K_a is the apparent dissociation constant of the EA_h1 complex, and h₁ is the Hill coefficient for the activation process. K_i is the apparent dissociation constant of the enzyme·l-aspartate complex (EAsp_h2), and h₂ is the Hill coefficient for the inhibition by l-aspartate.