. 2014 Oct 20;53(45):7100–7106. doi: 10.1021/bi501113u

Table 1. Kinetic Parameters for Acetyl-CoA Activation of the Pyruvate Carboxylation Reaction Catalyzed by RePC in the Presence of Different Concentrations of l-Aspartate^a.

[l-aspartate] (mM)	K_a for acetyl-CoA (μM)	Hill coefficient (h₁)	k_cat (s^–1)	k₀ (s^–1)
0	7.3 ± 0.9	2.47 ± 0.21	22.9 ± 0.5	0.32
2	14.5 ± 0.5	2.87 ± 0.26	23.9 ± 0.6	0.27
4	23.7 ± 0.7	2.48 ± 0.16	24.9 ± 0.6	0.23
6	35.6 ± 1.1	2.74 ± 0.22	22.4 ± 0.6	0.21
8	60.5 ± 3.0	2.54 ± 0.21	25.3 ± 1.2	0.19

Assay conditions: 0.1 M Tris-HCl (pH 7.8), 20 mM NaHCO₃, 6 mM MgCl₂, 1 mM MgATP, 0.2 mM NADH, 10 mM sodium pyruvate, and 5 units of malate dehydrogenase (MDH). The concentrations of acetyl-CoA and l-aspartate were varied from 0 to 150 μM and from 0 to 8 mM, respectively. The parameters (±standard errors) were estimated from a nonlinear regression fit of the data at each l-aspartate concentration to eq 1 except k₀, which was measured directly.

Table 1. Kinetic Parameters for Acetyl-CoA Activation of the Pyruvate Carboxylation Reaction Catalyzed by RePC in the Presence of Different Concentrations of l-Aspartatea.

Table 1. Kinetic Parameters for Acetyl-CoA Activation of the Pyruvate Carboxylation Reaction Catalyzed by RePC in the Presence of Different Concentrations of l-Aspartate^a.