Figure 2.

Tethering strategies employed in this study. For direct covalent attachment, proteins containing p-AzF were covalently attached to Sepharose beads functionalized with DBCO containing a 10-atom linker (scheme 1) or a 23-atom linker (scheme 2) as described in the Experimental Procedures and Supporting Information. For attachment mediated by biotin–streptavidin (schemes 3 and 4), proteins containing p-AcF or p-AzF were biotinylated and subsequently bound to Sepharose modified with streptavidin.