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. 2014 May 22;12(5):726–735. doi: 10.1111/jth.12542

Figure 4.

Figure 4

Alpha granule secretion, integrin activation and lysosome release are not affected by SecinH3. Washed platelets (4 × 107 mL−1) were treated with 0.2% DMSO vehicle (Veh) or 15 μm SecinH3 (SecH3) before stimulation for 10 min with a range of concentrations of α-thrombin (αT) (A, B) or CRP (D, E) in the presence of 1 mm CaCl2. P-selectin expression (A, D) as a result of α-granule secretion and integrin αIIbβ3 activation (B, E) were measured by flow cytometry. Data are shown as the percentage of maximal response. Alternatively, washed platelets (2 × 108 mL−1), treated with Veh or 15 μm SecH3, were stimulated for 15 min with αT (C) or CRP (F) in the presence of 1 mm CaCl2 and the release of β-hexosaminidase from lysosomes was measured by a colorimetric assay. Data are expressed as the percentage of total content. Curves (A–F) were fitted by F-test. Mean ± SEM, n ≥ 4.