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. 2014 May 22;12(5):748–760. doi: 10.1111/jth.12552

Figure 3.

Figure 3

IGF-1–stimulated TSC2 phosphorylation is insufficient to activate mTORC1. Washed platelets were stimulated with IGF-1 (100 nmol L−1) for the indicated times (A) or incubated with vehicle (0.2% DMSO), wortmannin (100 nmol L−1), MK-2206 (1 μmol L−1), BIM I (10 μmol L−1), or rapamycin (200 nmol L−1) for 15 min before stimulation with IGF-1 (100 nmol L−1) for 15 min (B). Phosphorylation of the indicated proteins was assessed by Western blotting of either whole cell lysates or immunoprecipitates (IP). Membranes were reprobed for α-tubulin to confirm equal loading.