Figure 5.

PMA stimulates TSC2 and p70SK phosphorylation independently of PI3 kinase/Akt. Washed platelets were stimulated with PMA (100 nmol L⁻¹) for the indicated times (A) or incubated with vehicle (0.2% DMSO), wortmannin (100 nmol L⁻¹), MK-2206 (1 μmol L⁻¹), BIM I (10 μmol L⁻¹), or AR-C 66096 (1 μmol L⁻¹) for 15 min before stimulation with PMA (100 nmol L⁻¹) for 15 min (B). Blots were performed on whole cell lysates with the exception of p70S6K which was immunoprecipitated from RIPA lysates. Membranes were reprobed for α-tubulin as a loading control.