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Factors affecting Mitrecin A activity. Residual activity of Mitrecin A was measured against Remazol brilliant blue (RBB)-labelled Y. pseudotuberculosis in quantitative dye-release assays at (a) various pH conditions (pH 4–11), (b) various saline concentrations (0–7·5%) and (c) after various 30-min temperature treatments (4–95°C). Residual activities represent the mean of three independent measurements.
