Figure 4.

Bacteriolytic activity of Mitrecin A. Residual viability of the Yersinia pseudotuberculosis indicator culture (a) was measured by determining the colony-forming units per millilitre after 16 h exposures of the culture to various concentrations of Mitrecin A. The regression coefficient (R²) of the plot is for the best-fit third-order polynomial. Microslide assays were performed to rapidly visualize the enzymatic activity of Mitrecin A against susceptible bacteria. In an illustration of the microslide assay (b), enzymatic activity for Mitrecin A (well 1, 1 μg), bovine serum albumin (well 2, 1 μg) and lysozyme (well 3, 1 μg) is shown against Aeromonas hydrophila.