Fig. 3.

Rice prolamin extract (RPE) inhibits the nuclear translocation of nuclear factor (NF)-κB/p65 in RIE and BMM. RIE (a) and BMM (b) were pretreated for 1 h with RPE (1000 μg/ml) and then stimulated with lipopolysaccharide (LPS) (10 μg/ml for RIE and 1 μg/ml for BMM) for 30 min. Nuclear translocation of NF-κB/p65 was visualized by immunofluorescence. Co-incubation of LPS with RPE inhibited the nuclear translocation of NF-κB/p65. RPE, rice prolamin extract; RIE, rat intestinal epithelial cell; BMM, bone marrow-derived macrophage.