Micrographs of new species in the third freshwater clade of subgenus Paraphysomonas: Paraphysomonas variosa, P. caroni, P. mantoni, P. aff. mantoni (Bassen).(A – F)P. variosa. (G – K)P. caroni. (M – L)P. mantoni. (O)P. aff. mantoni (Bassen). (A) DIC of live P. variosa cells, dividing (%) and with stalk (arrow). (B – F)P. variosa TEMs. (B) Close-up of tapering scale spine tip. (C) Whole cell with attached scales, possible malformed spines (double arrowhead). (D) Three detached scales showing diversity of size. (E) Close-up of tapering spine tips. (F) Attached scales showing detached mastigonemes or possibly malformed spines (double arrowhead) and example of shorter scale spine with wide inflated base (arrowhead). (G – J)P. caroni TEMs. (G) Whole cell with few cells attached (**) and long filaments, possibly discharged extrusomes (*). (H) Scales at surface of cell. (I) Scales at surface of cell showing slight oblique point at tapered spine tip. (J) Detached scale. (K) DIC of live P. caroni cells and contaminant (cnt.). Contaminant cells as food (arrow). (L) DIC of live P. mantoni cells, scale-base layer visible. (M,N)P. mantoni TEMs showing tapering scale tips and close-up of tapering spine tip (boxes). (O) DIC of live P. aff. mantoni (Bassen) cells and possible contaminant (cnt.). Scale bar: A, K, L and O, 5 μm. B and E, 0.5 μm. D, F, H, I, J, M and N, 1 μm. G, 2 μm.