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pH dependence of TDG activity for G.fC (Δ) and G.caC (O) substrates.91 The G.caC data is best fitted to a model for ionization of one essential protonated group (pKa1 = 5.75 ± 0.03) and a second group (pKa2 = 8.2 ± 0.7) that is not essential but confers higher activity when deprotonated (solid line). Fitting is poor for a model with a single essential protonated group (dotted line).
