Figure 4. Intestinal abnormalities in Nod2^−/− mice are observed in Rip2^−/− mice and dependent on Myd88.

(A–D) Quantification of Reg3β IF staining (A), number of goblet cells per villi (B) and proportion displaying abnormal morphology by light microscopy (C), and the proportion of IFN-γ⁺ IELs by flow cytometry (D) in the small intestine of WT and Rip2^−/− mice. (E) Representative IF staining of Reg3β in Nod2^−/− and Nod2^−/−MyD88^−/− small intestinal sections. Scale bar = 100 µm. (F) Quantification of Reg3β IF in WT, Nod2^−/−, MyD88^−/− and Nod2^−/−MyD88^−/−small intestine. (G) Quantification of the number of goblet cells per villi in WT, Nod2^−/−, MyD88^−/−and Nod2^−/−MyD88^−/− small intestine. (H) Flow cytometry analysis of the proportion of IFN-γ⁺ IELs in WT, Nod2^−/−, MyD88^−/− and Nod2^−/−MyD88^−/− mice. n ≥ 3 mice per genotype, **p<0.01 and ****p<0.0001 by unpaired two-tailed t test in (A), (B), (C), and (D), and ANOVA with a Holm-Sidak multiple comparisons test for (F), (G) and (H). All bar graphs display mean ± SEM from at least two independent experiments. Bars and numbers represent the mean in (B) and (G). See also figure S4