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. 2014 Nov 20;10(11):e1004777. doi: 10.1371/journal.pgen.1004777

Figure 1. sas-1 is a paternal-effect mutation required for bipolar spindle assembly in the embryo.

Figure 1

(A–B) Images from DIC time-lapse recording of wild type (A) or sas-1(t1476) (B) embryo. In this and other panels, time is indicated in min and sec, with t = 0:00 corresponding to pronuclear meeting. Red dots indicate MTOCs. See Movies S1 and S2. Schematics are shown to help interpret the corresponding DIC images. (C) Percentage of embryos of the indicated genotypes exhibiting monopolar spindle assembly in the first cell cycle. Note that the wild type was only imaged at 24°C. (D) Schematic representing the typical phenotype of one-cell stage embryos resulting from the joining of the indicated gametes. (E) Progeny test of indicated conditions. Although heterozygote sas-1(t1476) are not 100% viable, this likely reflects experimental variability, as the effect is weaker at the restrictive temperature. See also Table S1 and Fig. S1–S3.