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. 2014 Nov 20;10(11):e1004806. doi: 10.1371/journal.pgen.1004806

Figure 2. Tissue-specific heat-induced release of SDC gene silencing.

Figure 2

A, In vivo luciferase activity in transgenic seedlings. Top: Positive control UBQ3::LUC+ and transgenic dc mutant (-1200SDCProm::LUC+). Bottom: #6, #7, #8 and #9 represent independent Col-0 transgenic lines (-1200SDCProm::LUC+) under standard conditions or heat stress. B, Transcript levels of LUC+, SDC and HSP20 in different tissues of transgenic Col-0 seedlings (-1200SDCProm::LUC+) after a 5 heat cycle entrainment. Bars represent means ± SE as a log2 ratio with the non-treated control condition (i.e., control  = 0); replicated samples were pooled from 20–30 seedlings (E: epicotyl, H: hypocotyl). C, In vivo luciferase activity in 7-days-old Col-0 transgenic seedlings (-1200SDCProm::LUC+) after an entrainment of 5 heat-cycles and varying recovery times. LUC: luciferase signal, CHL: chlorophyll signal. Arrows depict specific leaves; co: cotyledon, and 1st, 2nd, 3th, 4th, 5th represent developing true leaves.