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. 2014 Nov 20;10(11):e1004810. doi: 10.1371/journal.pgen.1004810

Figure 3. Functional categorization of 250 genes depleted in ato.

Figure 3

(A) The ten genes that show the most signification depletion in ato, based on p-value. Each is color-coded according to the categories in panel (B). (B) Categorization of the 250 genes that show at least four-fold reduction in ato with a FDR <0.01. Putative functions were determined by examination of automated gene annotation and BLAST searches to identify similar proteins. (C) Gene Ontology (GO) term analysis using the program AmiGO identified four level two terms that were significantly enriched among the 250 ato-depleted genes compared to all D. melanogaster genes listed in FlyBase. See Figure S7 for the complete analysis of all level two GO terms. (D) Significance of enrichment of lower level terms, which form a subset of higher level terms (e.g. “detection of stimulus” is a subset of “response to stimulus”). Lower level terms were selected to illustrate the types of functions most enriched in the dataset. The number of ato-depleted genes annotated with each GO term is indicated. We note that the ion channel genes depleted in ato include two members of the ppk family, ppk25 and ppk10, two members of the Trp family, nanchung and inactive, and four potassium channel genes: shaw-like (shawl), TWIK-related acid-sensitive K+ channel (Task7), Inwardly rectifying potassium channel 1 (Irk1), and CG1756. Depleted genes described by “transmembrane signaling receptor activity” included six GPCRs: 5-HT2, 5-HT7, CG43795, frizzled 3 (fz3), Pigment-dispersing factor receptor (Pdfr), and CG18208.