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. 2014 Nov 20;10(11):e1004783. doi: 10.1371/journal.pgen.1004783

Figure 6. HAM-5-GFP and MAK-2-mCherry localize to puncta in Δham-7 and Δham-11 fusion-deficient germlings.

Figure 6

(A) Western blot of protein samples from 5 hr-old WT, Δham-5, Δham-7 and Δham-11 germlings probed with anti-p42/44 antibodies, which recognize phosphorylated MAK-1 and MAK-2 [8]. As previously shown [43], MAK-1 phosphorylation is reduced in the Δham-7 mutant. (B) HAM-5-GFP and MAK-2-mCherry localization in WT germlings undergoing chemotropic interactions. Arrows show localization to the CAT tip and to cytoplasmic puncta. (C) HAM-5-GFP and MAK-2-mCherry co-localization in Δham-7 (ham-5-gfp; mak-2-mCherry) germlings. Note lack of chemotropic interactions. Arrows show puncta of HAM-5-GFP and MAK-2-mCherry in Δham-7 (ham-5-gfp; mak-2-mCherry) germlings, which are often co-localized at the germ tube tip. (D) HAM-5-GFP and MAK-2-mCherry co-localization in Δham-11 (ham-5-gfp; mak-2-mCherry) germlings. Note lack of chemotropic interactions. HAM-5-GFP and MAK-2-mCherry co-localized to both cytoplasmic and tip-localized puncta (arrows). The upper left panels are bright field images, the upper right panels show GFP fluorescence, the lower left panels show mCherry fluorescence. Lower right panels show merged images of GFP and mCherry images. Scale bars  = 10 µM.