Figure 5. Tut and Bgcn are recruited by Bam to form a complex.

(A–B) S2 cells were transfected with the combinations of DNA constructs as indicated. Lysates from transfected S2 cells were immunoprecipitated with IgG (A) or anti-Myc (B) beads. Western blots were used to analyze the presence of TAP-, Flag-, or Myc-tagged proteins. Interaction between Tut and Bgcn was not detected in the absence of Bam. Asterisk in (A) indicates non-specific bands. (C–F) S2 cells were transfected with different combinations of DNA constructs as indicated. Lysates from transfected S2 cells were immunoprecipitated with anti-HA beads. Western blots were used to analyze the presence of Flag- or HA-tagged proteins. See also Figure S6.