Skip to main content
. 2014 Nov 20;10(11):e1004797. doi: 10.1371/journal.pgen.1004797

Figure 6. Tut is required for Bam to drive germline stem cell differentiation.

Figure 6

(A–B&B′) Genotype: UASp-bam-GFP/+; nos-Gal4/+. Forced expression of Bam in GSCs eliminated all germ cells. (C–D&D′) Genotype: UASp-bam-GFP/+; tut1 nos-Gal4/tut3. Germ cells were present in tut mutant background. Green dots outline the hub-adjacent germ cells expressing Bam-GFP. (E–E″) Immunofluorescence images of UASp-bam-GFP/+; tut1 nos-Gal4/tut3 testis. Green dots outline hub-adjacent germ cells expressing Bam-GFP and yellow arrowhead points to a dot-shape spectrosome. (F–F″) Immunofluorescence images of UASp-bam-GFP/+; tut1 nos-Gal4/tut3 testis. Green dots outline the hub-adjacent germ cells expressing Stat92E and Bam-GFP. (G&G′) 1 day old UASp-bam-GFP/+;tut1 nos-Gal4/tut3 testis labeled with BrdU for 1 hour. Green dotted-line highlights three hub-adjacent germ cells positive for Bam-GFP and two of them incorporated BrdU. (H) 10 day old UASp-bam-GFP/+;tut1 nos-Gal4/tut3 testis labeled with BrdU for 1 hour. Green dotted-line indicates two hub-adjacent germ cells positive for Bam-GFP and BrdU. Scale bars: 50 µm (A,C); 25 µm (B, D–H). See also Figure S7.