Figure 8. Loss of O-glucose on Eys results in its intracellular accumulation.

(A) Schematic of Eys^wt, Eys^1-4, in which four out of five Rumi target sites are mutated, and Eys^1-5, in which all Rumi target sites are mutated. (B–E′) Overexpression of Eys^1-5 (D,D′) and Eys^1-4 (E,E′), but not Eys^wt (B–C′), results in intracellular Eys accumulation (arrowheads). The GMR-GAL4 driver was used and all animals were raised at 18°C. Note that the GMR>eys^wt animal shown in (B,B′) and the GMR>eys^1-5 animal (D,D′) are younger than the GMR>eys^wt animal shown in (C,C′) and the GMR>eys^1-4 animal (E,E′). Scale bar: 5 µm.