Figure 4. 1MT treatment increases fungal loads, tissue pathology and mortality rates of susceptible but not resistant mice to P. brasiliensis infection.

Photomicrographs of lungs from 1MT treated and untreated A/J (A) and B10.A (B) mice at week 8 after infection with 1×10⁶ P. brasiliensis yeast cells obtained from groups of 5–6 mice. For panels: A and B, HE-stained, magnification ×10, Grocott-stained, magnification ×10. (C) Morphometric analysis of lung lesions of 1MT treated and untreated B10.A and A/J mice at week 8 post-infection (n = 5–6). (D) At weeks 2, 8 and 26 after infection, the presence of viable yeasts was determined in the liver of 1MT treated and untreated B10.A and A/J mice (n = 5–6). (E, F) Survival times of 1MT treated or untreated A/J and B10.A mice (9–10 mice per group) infected with 1×10⁶ P. brasiliensis yeasts were determined for a period of 250 days. The results are representative of two independent experiments (**p<0.01; ***p<0.001).