Figure 4. The active metabolite of Leflunomide, A771726, suppress virus production (A) and the expression of the pro-inflammatory cytokine IL-6 (B) in EV-A71-infected SF268 cells.
SF268 cells were infected with EV-A71 (moi 0.5), and various concentrations of A771726 were added to the infected cells after viral adsorption time. At 48 h post-infection, the culture supernatants and cell lysates were collected for virus titration (A). In a parallel experiment, the IL-6 protein levels in cell culture supernatant were measured using ELISA (B). Each virus stock to be titered is serially diluted in10-fold series (dilution fold 10−4) and added to Vero cells. Data are the mean ± s.e.m. from at least two parallel measurements per experiment.