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. 2014 Nov 20;9(11):e112930. doi: 10.1371/journal.pone.0112930

Figure 2. MiR-371-5p promoted the growth of pancreatic cancer cells in vitro and in vivo.

Figure 2

(A and B) The expression level of miR-371-5p was tested for 48 h in pancreatic cancer cells transfected with miR-371-5p mimics, anti-miR-371-5p and their respective control (NC, 40 nM) by qRT-PCR. (C and D) The proliferation of PDAC cell lines transiently transfected with miR-371-5p mimics, miR-371-5p inhibitor or control was analyzed by the MTT proliferation assay. (E) Anti-miR-371-5p increases the proportion of cells in G1 as estimated by using flow cytometry (left panel, **P<0.01, n = 4). And representative cell cycle histographs (anti-miR-NC vs anti-miR-371-5p) are presented (right panel). (F) Nude mice were subcutaneously inoculated with Panc-1 cells transfected with miR-371-5p plasmid or miR-NC control plasmid, in their flanks. The image is representative of tumors formed in 10 mice. (G) Growth curves of tumor volumes. The graph is representative of tumor growth, 5 weeks after inoculation. Tumor volume was calculated and all data are shown as the mean ± S.D. (n = 10).