Table 3. Effect of organic solvents on the stability of purified CD2-MnP from Irpex lacteus CD2.
Organic solvents | Concentration | Relative enzyme activity(%) | |
12 h | 24 h | ||
None | 100% | 100% | |
Methanol | 10% | 91.90 | 82.62 |
30% | 90.48 | 69.11 | |
Ethanol | 10% | 102.97 | 91.77 |
30% | 98.94 | 87.01 | |
DMSO | 10% | 94.87 | 90.54 |
30% | 94.04 | 80.68 | |
Ethylene glycol | 10% | 85.28 | 72.54 |
30% | 83.00 | 58.42 | |
Isopropyl alcohol | 10% | 85.34 | 77.24 |
30% | 29.21 | 10.24 | |
Butanediol | 10% | 134.74 | 119.33 |
30% | 129.13 | 84.42 | |
Glycerin | 10% | 92.83 | 85.41 |
30% | 84.96 | 80.50 | |
Acetonitrile | 10% | 95.24 | 80.02 |
30% | 26.72 | 22.35 |
The MnP activity of control without adding any organic solvent was set as 100%.