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. 2014 Nov 20;9(11):e113242. doi: 10.1371/journal.pone.0113242

Figure 3. Human umbilical vein endothelial cells (HUVECs) treated with tumor necrosis factor alpha (TNFα) and G15 induces inflammation.

Figure 3

(A, B) HUVECs were pretreated with PCA (100 µM) and G1 (3.0 µM) for 24 hrs. (A) treatment with TNFα (10 ng/ml) for 6 hrs. (B) treatment with TNFα (10 ng/ml) for 1 hr. (C) HUVECs were pre-treated with PCA (100 µM) for 24 hrs and then treated with G15 (3.0 µM) for 6 hrs. Blots are representative of 3 independent experiments. (A, B, C) Densitometric analyses are presented as the relative ratio of ICAM-1, VCAM-1, CD40, HIF-1α, GPER-1 or phospho-p38 to β-actin. (B) Densitometric analyses for phospho-NF-κB are presented as the relative ratio to NF-κB. Data are presented as mean±SEM; * P<0.05, ** P<0.005, *** P<0.001.