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. 2014 Nov 20;9(11):e113304. doi: 10.1371/journal.pone.0113304

Figure 2. Intermittent hypoxia enhanced CCR2 gene expression in monocytes.

Figure 2

THP-1 cells were treated with normoxia or intermittent hypoxia as described in methods. (A) RNA was isolated for the analysis of CCR2 gene expression by RT/real-time PCR. (B) Membrane proteins were prepared for western blot analysis. (C) Human peripheral monocytes were treated with the same conditions as in (A) and total RNA was isolated for the analysis of CCR2 gene expression by RT/real-time PCR. Data were present as means and standard errors from three independent experiments (mean ± SE, *: P<0.05 vs. Normoxia).