Figure 3. Up-regulation of monocytic CCR2 gene expression depended on the hypoxia level, but not TNF-α or CRP.

(A) THP-1 cells were treated with normoxia or intermittent hypoxia with different hypoxia levels (lowest O₂ set-point at 5% or 0.1%) as described in methods. RNA was isolated for the analysis of CCR2 mRNA expression by RT/real-time PCR. In the presence of TNF-α (B) or CRP (C), THP-1 cells were treated with intermittent hypoxia as described in methods. RNA was isolated for the analysis of CCR2 mRNA expression by RT/real-time PCR. (mean ± SE, *: P<0.05 vs. Normoxia, †: P<0.05 vs Normoxia, three independent experiments).