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. 2014 Nov 20;9(11):e113304. doi: 10.1371/journal.pone.0113304

Figure 6. Intermittent hypoxia induced the activation of p44/42 and p38 MAPK signal pathways in THP-1 cells.

Figure 6

THP-1 cells were treated with normoxia or intermittent hypoxia and cytosolic proteins were collected at 1, 3 and 6 hr for western blot analysis. The time-dependent activation of (A) p44/42 MAPK or (B) p38 MAPK through phosphorylation by intermittent hypoxia was investigated in THP-1 cells. Monocytes were pretreated with (C) PD98059 or (D) MSB202190 to inhibit p44/42 or p38 MAPK respectively for 1 hr and then the CCR2 mRNA expression was induced by intermittent hypoxia. (N: normoxia; IH: intermittent hypoxia) (mean ± SE, *: P<0.05 vs. Normoxia, †: P<0.05 vs Intermittent hypoxia + PD98059, ‡: P<0.05 vs Intermittent hypoxia + MSB202190).