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. 2014 Nov 20;9(11):e113262. doi: 10.1371/journal.pone.0113262

Figure 6. HDAC can repress the transcriptional activity of T/Bry via physical interaction.

Figure 6

(A) HDAC1 and HDAC3 interact with the T-box transcription factor T/Bry. Co-immunoprecipitation (Co-IP) was performed using control IgG or T/Bry antibody, followed by western blot analysis for HDAC1 and HDAC3. 5% Input (v/v) indicated that the ratio between the loading sample and precipitation is one to twenty. (B) Co-IP was performed using control IgG or HDAC1 antibody, followed by western blot analysis for T/Bry. (C) Co-IP was performed using control IgG or HDAC3 antibody, followed by western blot analysis for T/Bry. (D) HDAC3 does not interact with Gata4. Co-IP was performed using control IgG or HDAC3 antibody, followed by western blot analysis for Gata4. (E) A summary model shows the mechanism of HDACs in regulating the expression of mesodermal genes.