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. 2014 Nov 20;9(11):e113494. doi: 10.1371/journal.pone.0113494

Figure 2. Kis negatively affects growth of the presynaptic motor neuron but does not alter cytoskeletal proteins at the NMJ.

Figure 2

(A) Confocal images of third instar larval NMJs, muscles 6 and 7, labeled with α-HRP (white) to detect presynaptic neuronal membranes and phalloidin (red). Scale bar  = 20 µm (B) Quantification of muscle 6 sizes in w1118, kisk13416, and kisLM27/kisk13416. (C) Confocal micrographs of 6/7 NMJs from third instar larvae immunolabeled with α-HRP (magenta) and α-acetylated tubulin (green). Scale bar  = 20 µm (D) Quantification of mean relative synaptic acetylated tubulin levels (top histogram) and mean relative muscle acetylated tubulin levels (bottom histogram). (E) Quantification of total boutons (left histogram) and branches (right histogram) per 6/7 NMJ.