Figure 5. Shed GP induces the phenotypic maturation of DCs and MØ.
5×105 of DCs (A) and macrophages (B) were incubated with concentrated culture supernatants as above. The cells were harvested at 48 h post-incubation and expression of CD80, CD86, CD40 and CD83 was analyzed by flow cytometry. Filled histograms represent staining with an appropriate isotype-matched control antibody. Data shown are representative of three independent experiments using three blood donors. For quantitative data and statistics see figure S5.