Skip to main content
. 2014 Nov 20;10(11):e1004505. doi: 10.1371/journal.ppat.1004505

Figure 1. Cortical punctate structures that contain RCNMV MP are sites of viral RNA replication.

Figure 1

Nicotiana benthamiana protoplasts were inoculated with recombinant RCNMV RNAs that expressed the MP-mCherry fusion protein (Figure S1B) and subjected to immunostaining with anti-dsRNA primary antibody followed by Alexa Fluor 488-conjugated secondary antibody at an early stage of infection (16 hpi, left 2 rows of panels) and at a late stage of infection (24 hpi, center 2 rows of panels). The right-most panels show the results for mock-inoculated protoplasts treated with the same antibodies. Images present confocal projections of five optical sections at 1 µm intervals, which range from the surface to the middle of the protoplasts. DIC: differential interference contrast, N: nucleus. Scale bar = 20 µm.