Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Nov 20;10(11):e1004522. doi: 10.1371/journal.ppat.1004522

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Yao et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

(A) Domain illustration of TAB3 and summary of NleE interaction with TAB3 truncations. Interaction strength is scored by the number of + (also see the related data in Figure S4). CUE, CUE ubiquitin binding domain; TBD, TAK1-binding domain; CC, coiled-coil domain; NZF, Nlp4-like zinc finger domain. (B) Yeast two-hybrid assay of NleE interaction with TAB3 truncations. Yeast strain AH109 was transformed with plasmid combinations as illustrated (bait+prey). Plasmid combinations resulting in a positive interaction are highlighted in red. (C) Co-immunoprecipitation interaction assay of NleE and TAB3 truncations in transfected 293T cells. Shown are immunoblots of anti-Flag immunoprecipitates (Flag IP) and total cell lysates (Input).