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. 2014 Nov 20;10(11):e1004762. doi: 10.1371/journal.pgen.1004762

Figure 3. HAM-5 and MAK-2 dynamically co-localize in communicating germlings.

Figure 3

(A) Motile HAM-5-GFP puncta appear in a dynamic manner at opposing tips and in the cell body of two communicating germlings with an oscillation period of approximately three to five minutes and subsequently accumulate at the contact point of the two tips. See Video S2 for time course. Note that the diffuse cytoplasmic label of HAM-5-GFP decreased during the formation of HAM-5 puncta. Arrowheads indicate nuclei, from which HAM-5 is excluded. (B) HAM-5 and MAK-2 co-localize to puncta at the cell tip and within the germling (marked by arrows). MAK-2, but not HAM-5 also accumulates in nuclei (marked by arrowheads). Note that mCHERRY-tagged HAM-5 is strongly targeted to vacuoles for degradation (marked by red arrows). See Video S3 for time course. (C) Movement of a HAM-5/MAK-2 double-labeled spot towards the cell tip. See Video S3 for time course. (D) HAM-5 puncta can associate with nuclear envelopes (enlarged insert). See Video S4 for time course. (C) Movement of a HAM-5/MAK-2 double-labeled spot towards the cell tip. See Video S3 for time course.