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. 2014 Nov 20;10(11):e1004762. doi: 10.1371/journal.pgen.1004762

Figure 4. HAM-5 is a cell communication-specific scaffold of the MAK-2 module.

Figure 4

(A) Dynamic HAM-5-GFP oscillation is observed in approaching tips within the established mycelium. Dynamic localization of HAM-5 to septa is indicated by arrows, and septum-associated HAM-5-GFP puncta are enlarged in the first image (insert co-labeled with FM4-64 to show plasma membrane and septum). See Video S5 for time course. (B) HAM-5 is not enriched at hyphal tips of non-communicating hyphae, although HAM-5-GFP spots are visible throughout the cytoplasm of the growing hypha. Plasma membrane and Spitzenkörper are labeled with FM4-64. (C) HAM-5-GFP puncta are also formed in Δmak-2 hyphae (left panel) and germlings (right panel), although the protein does not concentrate at germling tips. Note the distinct localization patterns of HAM-5-GFP in the two contacting hyphae in a Δmak-2 colony: multiple HAM-5 puncta are visible the approaching (left) hypha, while primarily the septal pores are labeled in the contacted (right) hypha and only few puncta are visible. (D) Reciprocal co-immunoprecipitation experiments of the tagged kinases flag-NRC-1 and MAK-2-GFP indicate interaction of the MAPK module in wild type but not in the Δham-5 mutant.