Table 1. Mutant characteristics of MAK-2 pathway components.
| Strain (FGSC # or [reference]) | Annotation | Cell communication 1 2 | MAK-2 phosphorylation 2 |
| wild type (#987) | 95%±2 | 100% | |
| ΔNCU00455 (#17041×wt) | MAP3K regulator STE-50 | 0% | 0% |
| ΔNCU00627 (#16766) | hypothetical protein | 96%±2 | nd 3 |
| ΔNCU00772 [47] | STE kinase MST-1 | 95%±3 | 99%±10 |
| ΔNCU01440 (#11422) | type V myosin | 9%±3 * | 14%±8 |
| ΔNCU01789 (#15045) | IDC1 related protein HAM-5 | 9%±1 | 18%±9 |
| ΔNCU02606 (#17297) | hypothetical protein | 95%±2 | nd |
| ΔNCU02972 (#19746) | hypothetical protein | 95%±3 | nd |
| ΔNCU03124 (#17973 4) | casein kinase 2 alpha subunit CKA | 12%±4 | 28%±6 |
| ΔNCU05485 (#15567) | casein kinase 2 beta 1 subunit CKB-1 | 23%±2 * | 22%±8 |
| ΔNCU06265 (#11245) | hypothetical protein; HAM-13 | 33%±2 | 42%±5 |
| ΔNCU07280 (#13416 4) | protein kinase YPK-1 | 14%±2 * | nd |
| ypk-1(16-19) [79] | protein kinase YPK-1 | 54%±8 | 20%±8 |
| ΔNCU03616 (#12467) | Ras GTPase RAS-2/SMCO-7 | 11%±2 | 29%±10 |
| smco-7 [49] | Ras GTPase RAS-2/SMCO-7 | 9%±3 | 37%±7 |
| ΔNCU03894 (#11325) | STE kinase STE-20 | 77%±2 | 94%±5 |
| Δste-20;Δmst-1 (this study) | STE kinase double mutant | 69±5 | 93%±6 |
| ΔNCU06500 (#16014 4) | Ras GDP-GTP exchange factor | 14%±3 | 15%±9 |
| cdc-25(7-10) ([79]) | Ras GDP-GTP exchange factor | 5%±2 * | nd |
| Δste-20;Δras-2 (this study) | ste-20 ras-2 double mutant | 5%±3 | 21%±5 |
| ΔNCU08008 (#12371) | adenylate cyclase capping protein CAP-1 | 28%±5 | 63%±8 |
| ΔNCU08377 (#11514×wt) | adenylate cyclase CR-1 | 34%±5 * | 37%±6 |
| ΔNCU08957 (#19203) | hypothetical protein | 97%±2 | nd |
1
assayed at 6 h post inoculation except for strains marked with *, which were analyzed 8 h post inoculation due to reduced germination rate and abnormal growth.
2
in %±SEM.
3
not determined.
4
heterokaryotic deletion strain.