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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: J Immunol. 2014 Oct 27;193(11):5668–5677. doi: 10.4049/jimmunol.1302280

Figure 7. Inhibition of IL-27(p28) production by IL-10 is independent of SOCS3.

Figure 7

A. RT-PCR of C57BL/6J (Wt) macrophages for mRNA of SOCS3 after 6 h incubation with LPS (1 μg/ml) or IL-10 (10 ng/ml) alone or in combination. B. Relative inhibition of LPS-induced IL-27(p28) by IL-10 in macrophages derived from Wt mice or Tie2-Cre/SOCS3f1/f1 mice, 24h. C. Time course of the appearance of IL-27(p28) in cell culture supernatants of LPS-activated macrophages from Wt mice or Tie2-Cre/SOCS3f1/f1 mice in the absence or presence of IL-10. Data are representative of at least two independent experiments each in triplicates (A) or were done with n=4 mice per group (B, C). * P < 0.05, *** P < 0.001.