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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: J Immunol. 2014 Oct 27;193(11):5668–5677. doi: 10.4049/jimmunol.1302280

Figure 8. IL-10 limits IL-27(p28) production during polymicrobial sepsis and endotoxic shock.

Figure 8

A. Time course of IL-10 in plasma of Wt mice during endotoxic shock (LPS 10 mg/kg body weight i.p.), n=4-6/time point, ELISA. B. Wt mice were injected with neutralizing anti-IL-10R antibody (200 μg i.p., n=7) or isotype control IgG1κ (200 μg i.p., n=8) followed by endotoxemia and detection of circulating IL-27(p28) after 8 h, ELISA. C. IL-27(p28) in plasma during endotoxic shock in Wt mice (n=9) compared to IL-10-/- mice (n=6), 10 h. D. Detection of IL-27(p28) in plasma of Wt mice injected with neutralizing anti-IL-10R antibody (n=8) or control IgG1κ (n=7) followed by polymicrobial sepsis induced by CLP, 10 h. E. IL-27(p28) in plasma of Wt mice after sham surgery (n=6) or CLP (n=9) as compared to IL-10-/- mice after CLP (n=8), 10 h. F. Survival after CLP (‘mid-grade’) in Wt mice treated with control IgG and IL-10-/- mice treated with control IgG or neutralizing anti-IL-27(p28) antibody (40 μg/mouse i.p., n=12/group). Data were obtained using the numbers of mice per group as indicated. Antibodies were injected 1 h before LPS or CLP in all experiments. * P < 0.05, ** P < 0.01, *** P < 0.001; frame F: * P < 0.05 for IL-10-/-+Control IgG vs. IL-10-/-+anti-IL-27(p28), # P < 0.05 for Wt+Control IgG vs. IL-10-/-+Control IgG.