Extended Data Figure 3.

Catalytic activity of the D-16.12t enzyme. a, Ligation of two L-oligonucleotides on a L-RNA template, according to the reaction format shown in Fig. 1b. Reaction conditions: 0.05 μM enzyme-primer, 1 μM downstream substrate, 0.5 μM template, 250 mM MgCl₂, 250 mM NaCl, pH 8.5, 23 °C. b, Polymerization of L-GTP by extension of a L-oligonucleotide primer on a complementary L-RNA template (see Fig. 2b). Reaction conditions as above, but with no downstream substrate and with either 4 mM L-GTP (solid circles) or 4 mM each D- and L-GTP (open circles).