Fig. 3.
Time courses of changes in the expression of GABAA receptor subunit mRNAs that are primarily expressed in the LD and PO/VPM/VPL complex (α1, α4, β2, γ2 and δ). (a–c, f–h, k–m, p–r, u–w) On the left side we present representative images of film autoradiographs after in situ hybridization for the respective GABAA receptor subunit mRNAs. (d–e, i–j, n–o, s–t, x–y) Quantitative evaluation of relative optic densities (ROD) and respective statistics are shown on the right panels. ROD values are depicted as red (ipsilateral to TBI) and blue (contralateral to TBI) lines. Note the rapid decreases in most subunit mRNAs ipsilaterally. Interestingly, the mRNA expression remains mostly unchanged in the contralateral side. Significant increases were observed for the α4 subunit mRNA on the contralateral side at the late intervals and for δ subunit mRNA in both hemispheres after 6 h, respectively. Scale bar = 250 μm (a–c, f–h, k–m, p–r, u–w). Statistical significance: *p < 0.05, **p < 0.01, ***p < 0.001 as compared to controls. Abbreviations: LD, laterodorsal thalamic nucleus; PO, posterior thalamic nuclear group; VPM, ventroposteromedial nucleus; VPL, ventroposterolateral nucleus. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)