Supplementary Fig. 1.

Inhibition of PKA or PKG does not affect THDOC–mediated potentiation at α1β3γ2L GABA_A receptors. (A & C) Mean peak currents recorded for α1β3γ2L GABA_A receptors in response to EC₂₀ GABA or EC₂₀ GABA + 50 nM THDOC. Cells were either untreated or exposed to 500 nM myristoylated PKA inhibitor peptide 14-22 amide (PKAI; A) or 3 μM KT5823 (C). (B & D) Bar charts showing the potentiation of EC₂₀ GABA-activated currents by 50 nM THDOC in untreated cells (light grey bars; n = 6) or in treated cells before (black bars) and after (grey bars) 500 nM PKAI (B; n = 5) or 3 μM KT5823 (D; n = 6) treatment. *P < 0.05, paired t-test.