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. 2014 Nov 16;15:178. doi: 10.1186/1471-2369-15-178

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© Wang et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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TL1A induced IκBα degradation in HUVEC. (A) HUVEC transfected with mock vector or DR3 were treated with ascending concentration of TL1A for 30 minutes, IκBα showed a clear degradation after TL1A stimulation for the DR3-Flag transfected EC. Blots were representatives of 3 separate experiments. (B) Densitometry analysis showed a significant increase in IκBα degradation by TL1A at 0.2 and 2 μg/ml for the DR3 transfected EC compared to the mock transfected (13.3 ± 4 vs.4.3 ± 3.2, 29 ± 5.5 vs.7.2 ± 5.6 respectively) (p < 0.05). Values were expressed as percentages of reduction compared to TL1A 0 μg/ml IκBα densities. Results were mean ± SE from 3 experiments. (C, D) There was no difference in IκBα degradation after TNF treatment between HUVEC transfected with empty vector and DR3.