Figure 5.
VDR transactivation with supplemented SRC/p160 coactivators: analysis of VDR transactivation in the presence of overexpressed SRC1, GRIP1, and ACTR, both in the presence (+) and absence (−) of overexpressed RXRWT in response to 120 nM 1, 25D3. Data are represented as the average fold activation normalized to VDR alone ± SEM (n = 4). Asterisks above the bars indicate a significant increase in VDR transactivation compared with the VDR alone control. ***, P < .001; **, P < .01; *, P < .05. Asterisks above the brackets indicate a significant difference in transactivation between the presence and absence of RXRWT. The letter b indicates cotransfection with both RXRWT and SRC/p160 coactivators had a greater effect on VDR transactivation compared with VDR + RXRWT in the absence of an SRC/p160 construct (black bar). The letter a indicates that no difference in VDR transactivation was observed in the presence of both RXRWT and the indicated SRC coactivator vs. VDR + RXRWT in the absence of the SRC/p160 coactivator.