Figure 3.

5-aza-dC suppresses macrophage inflammation, migration, and adhesion. A–G, 5-Aza-dC inhibits LPS-stimulated (A–F) and stearate-stimulated (G) expression of proinflammatory genes in macrophages. Raw264.7 macrophages were pretreated with 5-aza-dC (0.5μM) for 4 days and then treated with LPS (100 ng/mL) (A–F) or stearate (C18:0, 250μM) (G) for 4 hours. Gene expression was measured by real-time RT-PCR and normalized to cyclophilin; n = 4 per group. H–I, 5-Aza-dC reduces macrophage adhesion to endothelial cells (H) and migration (I). BMDMs were pretreated with 0.5μM 5-Aza-dC for 4 days, and BMDM-endothelium adhesion or migration assays were performed as described in Materials and Methods; n = 5 per group. All data are expressed as mean ± SEM. *, P < .05; **, P < .01.