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. 2014 Sep 24;155(12):4925–4938. doi: 10.1210/en.2014-1595

Figure 4.

Figure 4.

5-Aza-dC inhibits macrophage ER stress. A and B, 5-Aza-dC inhibits ERN1/IRE-1α (A) and Chop (B) mRNA expression in macrophages. Raw264.7 macrophages were treated with 5-aza-dC (0.5μM) for up to 6 days. ERN1/IRE-1α (A) and Chop (B) mRNA expression was measured by real-time RT-PCR and normalized to cyclophilin; n = 4 per group. C–E, 5-Aza-dC inhibits phosphorylated (p-) and total ERN1/IRE-1α in macrophages. Raw264.7 macrophages were pretreated with 5-aza-dC (0.5μM) for 2 days and then were stimulated with stearate (C18:0, 250μM) for 1 day. Phosphorylated and total ERN1/IRE-1α was measured by immunoblotting. A representative blot is shown in C. The blots were quantitated for total (D) and phosphorylated (E) ERN1/IRE-1α expression with a Li-COR Odyssey infrared imager system and normalized to that of β-actin; n = 3 per group. All data are expressed as mean ± SEM. **, P < .01 (A and B); for D and E, groups labeled with different superscripts are statistically different from each other, P < .05.