Figure 7.

PPARγ and LXRα knockdowns substantially reverse the inhibitory effects of 5-aza-dC on inflammation and ER stress. A–F, PPARγ and LXRα knockdowns additively reverse the inhibitory effects of 5-aza-dC on TNF-α (A), IL-6 (B), CCL2 (C), CCL5 (D), IRE-1α (E), and Chop (F) mRNA expression in Raw264.7 macrophages. Raw264.7 macrophages were reversely transfected with PPARγ1 and/or LXRα siRNAs. Cells were then pretreated with 5-aza-dC (0.5μM) for 4 days. In A–D, cells were further stimulated with LPS (100 ng/mL) for 4 hours. Gene expression was measured by real-time RT-PCR and normalized to cyclophilin. All data are expressed as mean ± SE; n = 6 per group. Groups labeled with different letters are statistically significantly different, P < .05.